Ladies and gentlemen, here it is, as promised, part of my first set of reliable data!
 Here's what we did:
  The e.coli, staph. epidermidis, and lactobacillus were originally diluted 20 ug/mL, 20 ug/mL and 10 ug/mL, respectively (because Lactobacillus originally had better growth, so we needed to balance out the concentrations). After being put in the detergent, this cut the dilutions in half (10, 10 and 5 ug/mL).Since the lactobacillus was diluted down from 5 ug/mL, the second column represents that concentration of lacto. Then the third column would show that diluted in half, so 2.5 ug/mL, and so on.
To find the MIC, we had to take the control column (the last column) and divide it by 10. In the lactobacillus  case, that is about 0.11. Since the MIC is what happens when there is 10% growth, around 0.11 would be the number we're looking for.
This set of data is from the Lactobacillus MIC with Cascade (top three rows) and Dawn (bottom three rows).

2 3 4 5 6 7 8 9 10 11 0.058 0.058 0.058 0.058 0.058 0.059 0.059 0.059 0.059 0.059 0.36 0.981 1.046 1.113 1.163 1.124 1.132 1.078 1.156 1.222 0.447 0.944 0.949 0.973 1.027 1.096 1.083 1.024 1.072 1.164 0.376 1 0.952 0.94 0.978 1.031 1.011 0.988 1.077 1.074 0.072 0.081 0.102 0.408 0.426 0.926 1.047 1.004 1.027 1.081 0.37 0.117 0.038 0.083 0.436 0.877 1.085 1.006 1.129 1.078 0.536 0.096 0.238 1.093 0.417 0.66 0.76 1.061 1.201 1.123 0.057 0.054 0.055 0.059 0.064 0.06 0.058 0.06 0.059 0.059
Dilutions by column: 5 ug/mL   2.5 ug/mL  1.25 ug/mL...........AND SO ON.

 Below is the data for e.coli and staph. epidermidis, respectively, but the calculations show that neither detergent had much of an effect on them.

0.062	0.06	0.078	0.049	0.046	0.05	0.063	0.059	0.042	0.07
0.551	0.552	0.505	0.639	0.606	0.6	0.827	0.66	0.829	0.457
0.436	0.728	0.746	0.584	0.584	0.563	0.673	0.577	0.735	0.714
0.299	0.716	0.732	0.739	0.677	0.608	0.612	0.623	0.762	0.597
0.672	0.52	0.484	0.487	0.604	0.621	0.782	0.749	0.774	0.658
0.573	0.495	0.554	0.473	0.534	0.624	0.747	0.934	0.726	0.769
0.695	0.527	0.469	0.459	0.554	0.568	0.783	0.914	0.802	0.731
0.063	0.054	0.055	0.055	0.046	0.045	0.06	0.06	0.075	0.062
2	3	4	5	6	7	8	9	10	11
0.043	0.039	0.039	0.04	0.039	0.04	0.04	0.039	0.042	0.039
0.336	0.63	0.069	0.844	0.847	0.805	0.796	0.842	0.725	1.03
0.463	0.321	0.072	0.949	0.95	0.914	0.912	0.86	0.776	0.951
0.119	0.122	0.074	0.676	0.747	0.962	0.938	0.859	0.826	0.94
0.289	0.582	0.069	0.258	0.367	0.728	0.834	0.824	0.884	0.903
0.657	0.613	0.074	0.309	0.884	0.699	0.657	0.872	1.053	0.945
0.101	0.069	0.069	0.324	0.411	0.528	0.67	0.875	1.018	1.03
0.055	0.054	0.054	0.058	0.048	0.046	0.041	0.056	0.041	0.039

 10 ug/mL   5ug/mL   2.5ug/mL.......AND SO ON

 Since e.coli did not have these "magic numbers" for a true MIC, that means that the MIC for it would be much greater than the original concentration, so:

MICecoli= >10 ug/mL because it could take a lot more detergent to kill off the e.coli.


Since s. epidermidis responded slightly to the Dawn detergent (bottom 3 rows), its MIC would be 10->10 ug/mL.
And since Lactobacillus responded greatly to Dawn, its MIC would be 2.5 ug/mL, where the highlighted part of the table shows.

											0.066
											0.061

The purpose of an MIC is to determine the toxicity of a detergent- how many cells it kills. This test showed that Cascade was generally non-toxic to these gut flora (say Hallelujah, dishwasher lovers) and while the Dawn was generally non-toxic to e.coli and epidermidis, it was toxic to the Lactobacillus. Of course, these liquids were toxic at relatively high levels, so we are unsure of how much detergent a person would actually consume. One study by Mercurius- Taylor argued that it was somewhere around 100 ug/g weight.

But the more important thing is the biofilm tests. Gut flora accumulate in biofilms, rather than pockets of broth. Since the detergents didn't seem to kill the bacteria that much, it is quite possible that they just sweep up biofilms and carry them away. While this may not seem like a bad thing, if a colony of gut flora lives in one part of the intestine and is swept away to another part, it may overpopulate its new area and cause damage. So next week, I will be doing biofilm tests to see if these dish detergents remove the biofims as opposed to kill the bacteria inside  them.

 Пока Пока!! (Bye bye!!)

Mackenzie